SCON-a Short Conditional intrON for conditional knockout with one-step zygote injection.

Szu-Hsien Sam WuHeetak LeeRéka Szép-BakonyiGabriele ColozzaAyse BoeseKrista R GertNatalia HallayJi-Hyun LeeJihoon KimYi ZhuMargot M LinssenSandra Pilat-CarottaPeter HohensteinHans-Christian TheusslAndrea Pauli Bon-Kyoung Koo

Published in: Experimental & molecular medicine (2022)

The generation of conditional alleles using CRISPR technology is still challenging. Here, we introduce a Short Conditional intrON (SCON, 189 bp) that enables the rapid generation of conditional alleles via one-step zygote injection. In this study, a total of 13 SCON mouse lines were successfully generated by 2 different laboratories. SCON has conditional intronic functions in various vertebrate species, and its target insertion is as simple as CRISPR/Cas9-mediated gene tagging.

Keyphrases

crispr cas
genome editing
genome wide
gene expression
ultrasound guided
copy number
transcription factor
genome wide identification