Anti-Biofilm Effects of Z102-E of Lactiplantibacillus plantarum against Listeria monocytogenes and the Mechanism Revealed by Transcriptomic Analysis.
Jinyuan WeiXingguo ZhangMohamedelfatieh IsmaelQingping ZhongPublished in: Foods (Basel, Switzerland) (2024)
Lactic acid bacteria (LAB) are the most common probiotics, and they present excellent inhibitory effects on pathogenic bacteria. This study aimed to explore the anti-biofilm potential of the purified active substance of Lactiplantibacillus plantarum , named Z102-E. The effects of Z102-E on Listeria monocytogenes were investigated in detail, and a transcriptomic analysis was conducted to reveal the anti-biofilm mechanism. The results indicated that the sub-MIC of Z102-E (3.2, 1.6, and 0.8 mg/mL) decreased the bacterial growth and effectively reduced the self-aggregation, surface hydrophobicity, sugar utilization, motility, biofilm formation, AI-2 signal molecule, contents of extracellular polysaccharides, and extracellular protein of L. monocytogenes . Moreover, the inverted fluorescence microscopy observation confirmed the anti-biofilm effect of Z102-E. The transcriptomic analysis indicated that 117 genes were up-regulated and 214 were down-regulated. Z102-E regulated the expressions of genes related to L. monocytogenes quorum sensing, biofilm formation, etc. These findings suggested that Z102-E has great application potential as a natural bacteriostatic agent.
Keyphrases
- biofilm formation
- pseudomonas aeruginosa
- candida albicans
- staphylococcus aureus
- listeria monocytogenes
- escherichia coli
- cystic fibrosis
- genome wide
- lactic acid
- transcription factor
- artificial intelligence
- single molecule
- machine learning
- gene expression
- deep learning
- small molecule
- high throughput
- dna methylation
- bioinformatics analysis
- single cell
- binding protein
- climate change