Charge detection mass spectrometry on human-amplified fibrils from different synucleinopathies.
Aikaterini TsirkouFlora KaczorowskiMathieu VerdurandRana RaffoulJonathan PansieriIsabelle QuadrioFabien ChauveauRodolphe AntoinePublished in: Chemical communications (Cambridge, England) (2022)
Amyloid fibrils are self-assembled mesoscopic protein aggregates, which can accumulate to form deposits or plaques in the brain. In vitro amplification of fibrils can be achieved with real-time quaking-induced conversion (RT-QuIC). However, this emerging technique would benefit from a complementary method to assess structural properties of the amplification products. This work demonstrates the feasibility of nanospray-charge-detection-mass-spectrometry (CDMS) performed on α-synuclein (αSyn) fibrils amplified from human brains with Parkinson's disease (PD) or Dementia with Lewy bodies (DLB) and its synergistic combination with RT-QuIC.
Keyphrases
- mass spectrometry
- endothelial cells
- label free
- liquid chromatography
- high glucose
- induced pluripotent stem cells
- nucleic acid
- high resolution
- pluripotent stem cells
- loop mediated isothermal amplification
- real time pcr
- gas chromatography
- mild cognitive impairment
- high performance liquid chromatography
- parkinson disease
- white matter
- oxidative stress
- brain injury
- drug induced
- cognitive impairment
- subarachnoid hemorrhage
- quantum dots
- solar cells
- protein protein