Background. Hand hygiene is recognized as the leading measure to prevent cross-transmission of microorganisms. Regarding hospital acquired infections, the compliance of nurses with hand washing guidelines seems to be vital in preventing the disease transmission among patients. There is a paucity of studies exploring this subject in Asia. Especially medical and nursing student's knowledge of standard hand hygiene precautions is rarely compared. Methods. A cross-sectional study was conducted among 98 medical and 46 nursing students in a tertiary medical college in India. Knowledge was assessed using WHO hand hygiene questionnaire. Attitude and practices were evaluated by using another self-structured questionnaire. Z test was used to compare the percentage of correct responses between medical and nursing students. A P value less than 0.05 was considered significant. Results. Only 9% of participants (13 out of 144) had good knowledge regarding hand hygiene. Nursing students knowledge (P = 0.023) , attitude (P = 0.023), and practices (P < 0.05) were significantly better than medical students.