Effects of 7 T static magnetic fields on the expression of biological markers and the formation of bone in rats.
Sachiko Yamaguchi-SekinoTsutomu KiraMasaki SekinoManabu AkahanePublished in: Bioelectromagnetics (2018)
In this study, we aimed to evaluate the effects of 7 T static magnetic fields (SMFs) on rat mesenchymal stem cells (MSCs) in order to determine whether strong SMFs affected the osteogenesis of MSCs. MSCs were prepared from bone marrow cells obtained from the femurs of 7-week-old male Fischer 344 rats. MSCs were then combined with β-tricalcium phosphate (β-TCP), yielding two types of TCP/MSC constructs (TCP/P-1 and P-2) on day 0. Exposure was performed for 3 h/day for 6 days, and the experiments were performed twice using different exposure apparatus (cryovials or 4-well chambers) for each experiment. The results from gene expression, protein expression, and histological analyses showed no reproducible effects on both TCP/P-1 and TCP/P-2 MSC constructs, although osteocalcin levels for TCP/P-1 MSC constructs increased significantly once after 7 T exposure in two experiments. These findings contribute to understanding the effects of strong SMFs on MSC and osteoblasts. Bioelectromagnetics. 40:16-26, 2019. © 2018 Wiley Periodicals, Inc.
Keyphrases
- mesenchymal stem cells
- umbilical cord
- bone marrow
- gene expression
- cell therapy
- dna methylation
- poor prognosis
- induced apoptosis
- molecularly imprinted
- oxidative stress
- clinical trial
- stem cells
- cell cycle arrest
- cell proliferation
- signaling pathway
- binding protein
- endoplasmic reticulum stress
- high resolution
- bone loss