Pulsed Direct Current Arc-Induced Nanoelectrospray Ionization Mass Spectrometry.
Yuanji GaoMin ZhangHongru FengKaineng HuangBing XiaYuanjiang PanPublished in: Analytical chemistry (2024)
This study explores the innovative field of pulsed direct current arc-induced nanoelectrospray ionization mass spectrometry (DCAI-nano-ESI-MS), which utilizes a low-temperature direct current (DC) arc to induce ESI during MS analyses. By employing a 15 kV output voltage, the DCAI-nano-ESI source effectively identifies various biological molecules, including angiotensin II, bradykinin, cytochrome C, and soybean lecithin, showcasing impressive analyte signals and facilitating multicharge MS in positive- and negative-ion modes. Notably, results show that the oxidation of fatty acids using a DC arc produces [M + O - H] - ions, which aid in identifying the location of C═C bonds in unsaturated fatty acids and distinguishing between isomers based on diagnostic ions observed during collision-induced dissociation tandem MS. This study presents an approach for identifying the sn -1 and sn -2 positions in phosphatidylcholine using phosphatidylcholine and nitrate adduct ions, accurately determining phosphatidylcholine molecular configurations via the Paternò-Büchi reaction. With all the advantages above, DCAI-nano-ESI holds significant promise for future analytical and bioanalytical applications.
Keyphrases
- ms ms
- mass spectrometry
- angiotensin ii
- gas chromatography
- liquid chromatography
- fatty acid
- high glucose
- multiple sclerosis
- diabetic rats
- high performance liquid chromatography
- high resolution
- quantum dots
- capillary electrophoresis
- drug induced
- immune response
- nitric oxide
- angiotensin converting enzyme
- current status
- aqueous solution
- drinking water
- magnetic resonance
- gene expression
- machine learning
- artificial intelligence
- solid phase extraction
- water soluble
- simultaneous determination
- stress induced