Single cell transcriptomics identifies a unique adipose lineage cell population that regulates bone marrow environment.
Leilei ZhongLutian YaoRobert J TowerYulong WeiZhen MiaoJiwhan ParkRojesh ShresthaLuqiang WangWei YuNicholas HoldreithXiaobin HuangYejia ZhangWei TongYanqing GongJaimo AhnKatalin SusztakNathanial A DymentMingyao LiFanxin LongChider ChenPatrick SealeLing QinPublished in: eLife (2020)
Bone marrow mesenchymal lineage cells are a heterogeneous cell population involved in bone homeostasis and diseases such as osteoporosis. While it is long postulated that they originate from mesenchymal stem cells, the true identity of progenitors and their in vivo bifurcated differentiation routes into osteoblasts and adipocytes remain poorly understood. Here, by employing large scale single cell transcriptome analysis, we computationally defined mesenchymal progenitors at different stages and delineated their bi-lineage differentiation paths in young, adult and aging mice. One identified subpopulation is a unique cell type that expresses adipocyte markers but contains no lipid droplets. As non-proliferative precursors for adipocytes, they exist abundantly as pericytes and stromal cells that form a ubiquitous 3D network inside the marrow cavity. Functionally they play critical roles in maintaining marrow vasculature and suppressing bone formation. Therefore, we name them marrow adipogenic lineage precursors (MALPs) and conclude that they are a newly identified component of marrow adipose tissue.
Keyphrases
- single cell
- bone marrow
- adipose tissue
- mesenchymal stem cells
- rna seq
- insulin resistance
- high throughput
- young adults
- high fat diet induced
- umbilical cord
- high fat diet
- bone mineral density
- induced apoptosis
- stem cells
- cell therapy
- fatty acid
- oxidative stress
- genome wide
- postmenopausal women
- signaling pathway
- cell cycle arrest
- type diabetes
- gene expression
- cell death
- cell proliferation
- wild type
- childhood cancer