Unbiased proteomic mapping of the LINE-1 promoter using CRISPR Cas9.

Erica M BriggsPaolo MitaXiaoji SunSusan HaNikita VasilyevZev R LeopoldEvgeny NudlerJef D BoekeSusan K Logan

Published in: Mobile DNA (2021)

Our optimization and evaluation of gRNA specificity and application of the C-BERST method creates a tool for studying the regulatory mechanisms of LINE-1 in cancer. Further, we identified the dual specificity protein phosphatase, DUSP1, as a novel regulator of LINE-1 transcription.

Keyphrases

transcription factor
crispr cas
genome editing
papillary thyroid
dna methylation
high resolution
gene expression
structural basis
amino acid
protein protein