Matrix Stiffening and EGFR Cooperate to Promote the Collective Invasion of Cancer Cells.
Eloise M GrassetThomas BerteroAlexandre BozecJonas FriardIsabelle BourgetSabrina PisanoMargaux LecacheurMajdi MaielCaroline BailleuxAlexander EmelyanovVéronique HofmanPaul HofmanGuerrino MeneguzziChristophe DurantonDmitry V BulavinCédric GaggioliPublished in: Cancer research (2018)
In squamous cell carcinoma (SCC), tissue invasion by collectively invading cells requires physical forces applied by tumor cells on their surrounding extracellular matrix (ECM). Cancer-related ECM is composed of thick collagen bundles organized by carcinoma-associated fibroblasts (CAF) within the tumor stroma. Here, we show that SCC cell collective invasion is driven by the matrix-dependent mechano-sensitization of EGF signaling in cancer cells. Calcium (Ca2+) was a potent intracellular second messenger that drove actomyosin contractility. Tumor-derived matrix stiffness and EGFR signaling triggered increased intracellular Ca2+ through CaV1.1 expression in SCC cells. Blocking L-type calcium channel expression or activity using Ca2+ channel blockers verapamil and diltiazem reduced SCC cell collective invasion both in vitro and in vivo These results identify verapamil and diltiazem, two drugs long used in medical care, as novel therapeutic strategies to block the tumor-promoting activity of the tumor niche.Significance: This work demonstrates that calcium channels blockers verapamil and diltiazem inhibit mechano-sensitization of EGF-dependent cancer cell collective invasion, introducing potential clinical strategies against stromal-dependent collective invasion.Graphical Abstract: http://cancerres.aacrjournals.org/content/canres/78/18/5229/F1.large.jpg Cancer Res; 78(18); 5229-42. ©2018 AACR.
Keyphrases
- extracellular matrix
- cell migration
- squamous cell carcinoma
- small cell lung cancer
- induced apoptosis
- poor prognosis
- single cell
- cell cycle arrest
- epidermal growth factor receptor
- bone marrow
- cell therapy
- mental health
- tyrosine kinase
- growth factor
- cell proliferation
- oxidative stress
- cell death
- radiation therapy
- reactive oxygen species
- binding protein
- young adults
- signaling pathway
- climate change
- angiotensin ii
- anti inflammatory