DNA Extraction with DNAzol and LAMP, Performed in a Heating Block as a Simple Procedure for Detection of Mycobacterium tuberculosis in Sputum Specimens.
Álvaro Rodríguez-GarcíaRosa E MaresPatricia L A MuñozSamuel G Meléndez-LópezAlexei Fedorovish Licea-NavarroMarco A Ramos-IbarraPublished in: Methods and protocols (2018)
Tuberculosis (TB) remains as a major public health issue in developing countries. Accurate detection is essential for the proper management of patients with active disease. Here, we present a simple DNAzol-LAMP (loop-mediated isothermal amplification) procedure for the detection of Mycobacterium tuberculosis in sputum specimens. Twenty smear-positive sputum samples were analyzed as follows: (i) Genetic material was extracted by a standard DNAzol protocol, and (ii) mycobacterial DNA was detected by a typical TB-specific loop-mediated isothermal amplification method. Results and diagnostic test performance attests to the suitability of the proposed procedure.
Keyphrases
- loop mediated isothermal amplification
- mycobacterium tuberculosis
- pulmonary tuberculosis
- sensitive detection
- public health
- minimally invasive
- circulating tumor
- single molecule
- cell free
- randomized controlled trial
- emergency department
- cystic fibrosis
- quantum dots
- mass spectrometry
- human immunodeficiency virus
- label free