Inhibitory effects of Orostachys malacophyllus var. iwarenge extracts on reactive oxygen species production and lipid accumulation during 3T3-L1 adipocyte differentiation.
Miran JangHye-Young ChoiHwan Hee LeePublished in: Food science and biotechnology (2018)
Orostachys malacophyllus var. iwarenge was extracted with water, 30 and 70% ethyl alcohol. The ethyl alcohol extracts showed higher total phenol contents and greater antioxidant effects than the water extract. Treatment with the 70% alcohol extract inhibited reactive oxygen species (ROS) production and lipid accumulation during 3T3-L1 adipogenesis. Furthermore, the 70% extract inhibited the mRNA and protein expressions of the pro-oxidant enzyme NADPH oxidase 4 and of the NADPH-producing enzyme glucose-6-phosphate dehydrogenase. The mRNA and protein expressions of antioxidant enzymes, such as copper-zinc superoxide dismutase and manganese superoxide dismutase increased in cells treated with the 70% alcohol extract. In addition, this extract suppressed the mRNA and protein levels of adipogenic transcription factors and of their marker genes. These results indicate that O. malacophyllus extracts inhibit lipid accumulation and ROS production by controlling adipogenic factors and pro-/anti-oxidant enzyme responses.
Keyphrases
- anti inflammatory
- reactive oxygen species
- oxidative stress
- binding protein
- alcohol consumption
- induced apoptosis
- dna damage
- cell death
- transcription factor
- amino acid
- hydrogen peroxide
- ionic liquid
- adipose tissue
- oxide nanoparticles
- fatty acid
- insulin resistance
- blood pressure
- signaling pathway
- genome wide
- skeletal muscle
- combination therapy
- cell cycle arrest
- genome wide identification