Factors influencing biased agonism in recombinant cells expressing the human α1A -adrenoceptor.

Edilson Dantas da Silva JuniorMasaaki SatoJon MerlinNatalie BroxtonDana S HutchinsonSabatino VenturaBronwyn A EvansRoger J Summers

Published in: British journal of pharmacology (2017)

We have shown that while adrenergic agonists display bias at human α1A -adrenoceptors, the marked bias of oxymetazoline for ERK1/2 phosphorylation originates from off-target effects. Commonly used cell lines express a repertoire of endogenous GPCRs that may confound studies on biased agonism at recombinant receptors.

Keyphrases

endothelial cells
induced pluripotent stem cells
induced apoptosis
pluripotent stem cells
signaling pathway
cell proliferation
oxidative stress
cell death
endoplasmic reticulum stress