Production of isotope-labeled proteins in insect cells for NMR.
Bastian FrankeChristian OpitzShin IsogaiAnne GrahlLeonildo DelgadoAlvar D GossertStephan GrzesiekPublished in: Journal of biomolecular NMR (2018)
Baculovirus-infected insect cells have become a powerful tool to express recombinant proteins for structural and functional studies by NMR spectroscopy. This article provides an introduction into the insect cell/baculovirus expression system and its use for the production of recombinant isotope-labeled proteins. We discuss recent advances in inexpensive isotope-labeling methods using labeled algal or yeast extracts as the amino acid source and give examples of advanced NMR applications for proteins, which have become accessible by this eukaryotic expression host.
Keyphrases
- induced apoptosis
- poor prognosis
- cell cycle arrest
- magnetic resonance
- pet imaging
- high resolution
- amino acid
- aedes aegypti
- single cell
- endoplasmic reticulum stress
- gas chromatography
- cell death
- solid state
- signaling pathway
- mesenchymal stem cells
- binding protein
- pi k akt
- long non coding rna
- cell free
- zika virus
- bone marrow
- tandem mass spectrometry