Anti-Inflammatory Salicin Derivatives from the Barks of Salix tetrasperma .
Pei-Qian WuYing LiYu-Hao RenJun-Su ZhouQun-Fang LiuYan WuJin-Hai YuBin ZhouJian-Min YuePublished in: Journal of agricultural and food chemistry (2024)
The genus Salix L. is traditionally used in folk medicine to alleviate pain caused by various kinds of inflammation. In the present study, 10 undescribed salicin derivatives along with 5 known congeners were isolated from the barks of Salix tetrasperma , and their structures were elucidated by spectroscopic analyses, single-crystal X-ray diffraction, electronic circular dichroism (ECD) calculations, and chemical conversions. Compounds 4 - 6 significantly inhibited NO production in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages, and the most active 4 obviously suppressed the production of IL-1β and IL-6 and decreased iNOS and COX-2 expression in a dose-dependent manner. Further Western blotting analysis revealed that the anti-inflammatory mechanism of 4 is possibly mediated through the MAPK and NF-κB signaling pathways.
Keyphrases
- lps induced
- anti inflammatory
- inflammatory response
- signaling pathway
- oxidative stress
- pi k akt
- high resolution
- poor prognosis
- chronic pain
- molecular docking
- molecular dynamics
- pain management
- molecular dynamics simulations
- density functional theory
- toll like receptor
- magnetic resonance
- single cell
- south africa
- electron microscopy
- mass spectrometry
- nitric oxide synthase
- immune response
- spinal cord
- computed tomography
- nuclear factor
- contrast enhanced