5-Aminolevulinic acid hydrochloride loaded microbubbles-mediated sonodynamic therapy in pancreatic cancer cells.
Wei YangHaitao XuQinghao LiuChunxun LiuJiahe HuPing LiuTianyi FangYuxian BaiJiuxin ZhuRui XiePublished in: Artificial cells, nanomedicine, and biotechnology (2021)
5-Aminolevulinic acid hydrochloride (ALA)-mediated sonodynamic therapy (SDT) had anti-tumour effect on pancreatic cancer cells. Hence, ALA loaded lipid/poly(lactic-co-glycolic acid) (PLGA) microbubbles (MBs)-mediated SDT for pancreatic cancer has great potential. The average size of ALA-lipid MBs and ALA-PLGA MBs was about 3.0 µm. The two kinds of MBs had good biocompatibility to normal HPDE6-C7 cells and were not toxic to pancreatic cancer cells. Compared with ALA-induced SDT, a statistically significant decrease in cell viability was observed in ALA lipid/PLGA MBs combined with ultrasound groups in AsPC-1 and BxPC-3 cells (p < .05). Obvious effect on the apoptotic rate, apoptosis and pyroptosis morphology, enhanced reactive oxygen species was found in ALA-lipid/PLGA MBs mediated SDT in vitro. Through in vivo study, we found ALA-lipid/PLGA MBs-mediated SDT was a promise treatment for pancreatic cancer.
Keyphrases
- drug delivery
- cell cycle arrest
- induced apoptosis
- fatty acid
- cell death
- drug release
- photodynamic therapy
- reactive oxygen species
- oxidative stress
- endoplasmic reticulum stress
- magnetic resonance imaging
- stem cells
- bone regeneration
- signaling pathway
- computed tomography
- cell proliferation
- high glucose
- diabetic rats
- mesenchymal stem cells
- cell therapy
- climate change
- ultrasound guided
- drug induced