JC polyomavirus modifies the expression of human microRNAs in PML brain.
Anni HonkimaaJoni SuppulaOlli TynninenMika SaarelaHanna LiimatainenPia LainePetri AuvinenEeva AuvinenPublished in: The Journal of infectious diseases (2023)
Progressive multifocal leukoencephalopathy (PML) is a severe neurological condition caused by reactivation of JC polyomavirus (JCPyV) in immunosuppression. Asymptomatic JCPyV persists in peripheral tissues. Upon reactivation, neurotropic rearrangements may emerge, and the virus gains access to the brain. To assess the mechanisms of PML pathogenesis, brain tissue material from PML patients was collected for small RNA sequencing. Upregulation of eight microRNAs (miRNAs) in PML brain was validated using quantitative microRNA PCR. Bioinformatics tools were utilized to identify major associations of the upregulated miRNAs: neuroinflammation and blood-brain-barrier disruption. The results indicate involvement of human miRNA regulation in PML pathogenesis.
Keyphrases
- cerebral ischemia
- blood brain barrier
- resting state
- white matter
- endothelial cells
- functional connectivity
- poor prognosis
- end stage renal disease
- multiple sclerosis
- newly diagnosed
- subarachnoid hemorrhage
- brain injury
- ejection fraction
- signaling pathway
- cell proliferation
- chronic kidney disease
- gene expression
- traumatic brain injury
- single cell
- high resolution
- early onset
- peritoneal dialysis
- mass spectrometry
- long non coding rna