A highly sensitive and specific Homo1-based real-time qPCR method for quantification of human umbilical cord mesenchymal stem cells in rats.

Jing HeZhangfan WangChunchun AoChengshu TuYaqi ZhangCheng ChangCuihong XiaoE XiangWei RaoChangyong LiDongcheng Wu

Published in: Biotechnology journal (2024)

In this study, we establish a highly sensitive and specific Homo1-based real-time quantitative PCR method to quantify the distribution of human UC-MSCs in rats. The method provides guidelines for the safety research of cells in preclinical stages.

Keyphrases

mesenchymal stem cells
umbilical cord
endothelial cells
cell therapy
bone marrow
induced apoptosis
pluripotent stem cells
clinical practice
living cells
cell cycle arrest
fluorescent probe
cell proliferation
endoplasmic reticulum stress
signaling pathway
liquid chromatography
tandem mass spectrometry
real time pcr