Validation of a new multiparametric protocol to assess viability, acrosome integrity and mitochondrial activity in cooled and frozen thawed boar spermatozoa.
Raul A Gonzalez-CastroFernando J PeñaLisa A HerickhoffPublished in: Cytometry. Part B, Clinical cytometry (2022)
Our results indicate that a four-color protocol is a practical, simple and reliable procedure to simultaneously evaluate boar sperm viability, acrosome integrity and mitochondrial activity under clinical conditions.