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Spatial-CITE-seq: spatially resolved high-plex protein and whole transcriptome co-mapping.

Rong FanYang LiuMarcello DiStasioGraham SuHiromitsu AsashimaArchibald EnninfulXiaoyu QinYanxiang DengPino BordignonMarco CassanoMary TomaykoMina XuStephanie HaleneJoseph E CraftDavid A Hafler
Published in: Research square (2022)
We present spatial-CITE-seq for high-plex protein and whole transcriptome co-mapping, which was firstly demonstrated for profiling 198 proteins and transcriptome in multiple mouse tissue types. It was then applied to human tissues to measure 283 proteins and transcriptome that revealed spatially distinct germinal center reaction in tonsil and early immune activation in skin at the COVID-19 mRNA vaccine injection site. Spatial-CITE-seq may find a range of applications in biomedical research.
Keyphrases
  • single cell
  • rna seq
  • genome wide
  • high resolution
  • gene expression
  • dna methylation
  • binding protein
  • sars cov
  • endothelial cells
  • coronavirus disease
  • protein protein
  • high density
  • ultrasound guided