Identification of Matrix Metalloproteinase-1-Suppressive Peptides in Feather Keratin Hydrolysate.
Hyeon-Su JinKyeongseop SongJe-Hyun BaekJae-Eun LeeDa Jeong KimGae-Won NamNam Joo KangDong-Woo LeePublished in: Journal of agricultural and food chemistry (2018)
Inhibition of matrix metalloproteinases (MMPs), which degrade collagen and elastin in the dermis of normal skin, is a key strategy for anti-skin aging. In this study, we identified five low-molecular-weight (LMW, <1 kDa) MMP-1-suppressive peptides in feather keratin hydrolysate (FKH) obtained by anaerobic digestion with an extremophilic bacterium. FKH was first subjected to ultrafiltration, followed by size-exclusion chromatography and liquid chromatography/electrospray ionization tandem mass spectrometry analysis. Chemically synthesized peptides identical to the sequences identified suppressed MMP expression in human dermal fibroblasts (HDFs). To investigate the impact of the MMP-1-suppressive peptides on the signaling pathway, we performed antibody array phosphorylation profiling of HDFs. The results suggested that the peptide GGFDL regulates ultraviolet-B-induced MMP-1 expression by inhibiting mitogen-activated protein kinases and nuclear factor κB signaling pathways as well as histone modification. Thus, LMW feather keratin peptides could serve as novel bioactive compounds to protect the skin against intrinsic and extrinsic factors.
Keyphrases
- tandem mass spectrometry
- liquid chromatography
- signaling pathway
- ultra high performance liquid chromatography
- mass spectrometry
- nuclear factor
- high performance liquid chromatography
- wound healing
- anaerobic digestion
- poor prognosis
- simultaneous determination
- amino acid
- high resolution mass spectrometry
- high resolution
- gas chromatography
- cell migration
- soft tissue
- pi k akt
- toll like receptor
- endothelial cells
- solid phase extraction
- dna methylation
- induced apoptosis
- drug induced
- heat shock protein
- immune response
- binding protein
- high speed
- induced pluripotent stem cells
- risk assessment