13C-labeling reveals non-conventional pathways providing carbon for hydroxy fatty acid synthesis in Physaria fendleri.

Physaria fendleri is a Brassicaceae that produces hydroxy fatty acids in its embryos; a type of oil that is very valuable and widely used in the industry of cosmetics, lubricants, biofuels, etc. Free of toxins and rich in hydroxy fatty acids, Physaria is a promising alternative to imported castor oil and is on the verge of being commercialized. This study aims to identify important biochemical step(s) for oil synthesis in Physaria, which may serve as target(s) for future crop improvement. To advance towards this goal, the endosperm composition was analyzed by LC-MS/MS to develop and validate culture conditions that mimic the development of the embryos in planta. Using developing Physaria embryos in culture and 13C-labeling, our studies revealed that: i) Physaria embryos metabolize carbon into biomass with an efficiency significantly lower than other photosynthetic embryos; ii) the plastidic malic enzyme provides 42% of the pyruvate used for de novo fatty acid synthesis, which is the highest measured so far in developing "green" oilseed embryos; and iii) Physaria uses non-conventional pathways to channel carbon into oil, namely the RuBisCO shunt which fixes CO2 released in the plastid, and the reversibility of the isocitrate dehydrogenase which provides additional carbon for fatty acid elongation.