Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9.

Dongdong ZhaoShenli YuanBin XiongHongnian SunLijun YeJing LiXueli ZhangZaiqiang Wu

Published in: Microbial cell factories (2016)

In this study, we developed a fast and easy genome editing procedure based on CRISPR/Cas9 system that only required the work of one plasmid construction and one transformation, which allowed modification of a chromosome locus within 3 days and could be performed continuously for multiple loci.

Keyphrases

crispr cas
genome editing
escherichia coli
genome wide association study
genome wide
copy number
cystic fibrosis