Retrospective Detection and Complete Genomic Sequencing of Canine morbillivirus in Eurasian Otter ( Lutra lutra ) Using Nanopore Technology.
Zsófia LanszkiJózsef LanszkiGábor Endre TóthSafia ZeghbibFerenc JakabGábor KemenesiPublished in: Viruses (2022)
The Eurasian otter ( Lutra lutra ) is a piscivorous apex predator in aquatic habitats, and a flagship species of conservation biology throughout Europe. Despite the wide distribution and ecological relevance of the species, there is a considerable lack of knowledge regarding its virological and veterinary health context, especially in Central Europe. Canine morbillivirus (Canine distemper virus (CDV)) is a highly contagious viral agent of the family Paramyxoviridae with high epizootic potential and veterinary health impact. CDV is present worldwide among a wide range of animals; wild carnivores are at particular risk. As part of a retrospective study, lung-tissue samples ( n = 339) from Eurasian otters were collected between 2000 and 2021 throughout Hungary. The samples were screened for CDV using a real-time RT-PCR method. Two specimens proved positive for CDV RNA. In one sample, the complete viral genome was sequenced using a novel, pan-genotype CDV-specific amplicon-based sequencing method with Oxford Nanopore sequencing technology. Both viral sequences were grouped to a European lineage based on the hemagglutinin-gene phylogenetic classification. In this article, we present the feasibility of road-killed animal samples for understanding the long-term dynamics of CDV among wildlife and provide novel virological sequence data to better understand CDV circulation and evolution.
Keyphrases
- single cell
- healthcare
- sars cov
- public health
- human health
- mental health
- single molecule
- risk assessment
- genetic diversity
- copy number
- hiv infected
- health information
- deep learning
- antiretroviral therapy
- electronic health record
- cross sectional
- real time pcr
- dna methylation
- loop mediated isothermal amplification
- health promotion
- ultrasound guided
- genome wide identification
- fine needle aspiration