Multiphoton intravital microscopy of rodents.
Colinda L G J ScheeleDavid HerrmannErika YamashitaCristina Lo CelsoCraig N JenneMaja H OktayDavid EntenbergPeter FriedlRoberto WeigertFranck L B MeijboomMasaru IshiiPaul TimpsonJacco van RheenenPublished in: Nature reviews. Methods primers (2022)
Tissues are heterogeneous with respect to cellular and non-cellular components and in the dynamic interactions between these elements. To study the behaviour and fate of individual cells in these complex tissues, intravital microscopy (IVM) techniques such as multiphoton microscopy have been developed to visualize intact and live tissues at cellular and subcellular resolution. IVM experiments have revealed unique insights into the dynamic interplay between different cell types and their local environment, and how this drives morphogenesis and homeostasis of tissues, inflammation and immune responses, and the development of various diseases. This Primer introduces researchers to IVM technologies, with a focus on multiphoton microscopy of rodents, and discusses challenges, solutions and practical tips on how to perform IVM. To illustrate the unique potential of IVM, several examples of results are highlighted. Finally, we discuss data reproducibility and how to handle big imaging data sets.
Keyphrases
- single molecule
- high resolution
- gene expression
- high speed
- high throughput
- optical coherence tomography
- immune response
- big data
- label free
- single cell
- electronic health record
- induced apoptosis
- cell therapy
- cell cycle arrest
- stem cells
- machine learning
- dendritic cells
- artificial intelligence
- inflammatory response
- photodynamic therapy
- data analysis
- fluorescence imaging
- human health