Fast gene disruption in Trichoderma reesei using in vitro assembled Cas9/gRNA complex.

Published in: BMC biotechnology (2019)

Direct transformation of Cas9/gRNA complex into the cell is a fast means to disrupt a gene in T. reesei and may find wide applications in strain improvement and functional genomics study.

Keyphrases

crispr cas
genome editing
single cell
copy number
genome wide
genome wide identification
cell therapy
stem cells
gene expression
transcription factor
bone marrow