Single-Cell Mechanophenotyping in Microfluidics to Evaluate Behavior of U87 Glioma Cells.
Esra SengulMeltem ElitasPublished in: Micromachines (2020)
Integration of microfabricated, single-cell resolution and traditional, population-level biological assays will be the future of modern techniques in biology that will enroll in the evolution of biology into a precision scientific discipline. In this study, we developed a microfabricated cell culture platform to investigate the indirect influence of macrophages on glioma cell behavior. We quantified proliferation, morphology, motility, migration, and deformation properties of glioma cells at single-cell level and compared these results with population-level data. Our results showed that glioma cells obtained slightly slower proliferation, higher motility, and extremely significant deformation capability when cultured with 50% regular growth medium and 50% macrophage-depleted medium. When the expression levels of E-cadherin and Vimentin proteins were measured, it was verified that observed mechanophenotypic alterations in glioma cells were not due to epithelium to mesenchymal transition. Our results were consistent with previously reported enormous heterogeneity of U87 glioma cell line. Herein, for the first time, we quantified the change of deformation indexes of U87 glioma cells using microfluidic devices for single-cells analysis.
Keyphrases
- single cell
- high throughput
- rna seq
- signaling pathway
- induced apoptosis
- stem cells
- biofilm formation
- poor prognosis
- adipose tissue
- electronic health record
- endothelial cells
- oxidative stress
- big data
- mesenchymal stem cells
- cystic fibrosis
- single molecule
- artificial intelligence
- binding protein
- endoplasmic reticulum stress
- cell proliferation
- machine learning
- pseudomonas aeruginosa