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In vivo monitoring of plant small GTPase activation using a Förster resonance energy transfer biosensor.

Hann Ling WongAkira AkamatsuQiong WangMasayuki HiguchiTomonori MatsudaJun OkudaKen-Ichi KosamiNoriko InadaTsutomu KawasakiTakako Kaneko-KawanoShingo NagawaLi TanYoji KawanoKo Shimamoto
Published in: Plant methods (2018)
Our protocol should be adaptable for monitoring activation for other plant small GTPases and protein-protein interactions for other FRET sensors in various plant cells.
Keyphrases
  • energy transfer
  • quantum dots
  • induced apoptosis
  • randomized controlled trial
  • cell wall
  • cell cycle arrest
  • single molecule
  • living cells
  • plant growth
  • pi k akt
  • fluorescent probe