Gene Expression Analysis of Immunomagnetically Enriched Circulating Tumor Cell Fraction in Castration-Resistant Prostate Cancer.
Markéta ŠkereňováVeronika MikulováOtakar ČapounDavid ŠvecKatarína KološtováViktor SoukupHana HonováTomáš HanušTomáš ZimaPublished in: Molecular diagnosis & therapy (2019)
Detection of AR expression by AdnaTest® in CTC-enriched samples may help predict patients' survival. These AdnaTest® CTC-enriched samples can be used in a high-throughput quantitative polymerase chain reaction (qPCR) analysis of gene expression, provided that the specificity of the assay for each individual gene is properly validated. The BioMark™ platform can be used for the simultaneous detection of ARFL and ARV7 and other genes in CTC-enriched samples from CRPC patients.
Keyphrases
- gene expression
- high throughput
- end stage renal disease
- circulating tumor
- circulating tumor cells
- newly diagnosed
- ejection fraction
- chronic kidney disease
- dna methylation
- peritoneal dialysis
- prognostic factors
- poor prognosis
- single cell
- high resolution
- transcription factor
- patient reported outcomes
- mesenchymal stem cells
- mass spectrometry
- bone marrow
- label free
- free survival
- quantum dots