In vitro potential of human mesenchymal stem cells for corneal epithelial regeneration.
Núria Nieto-NicolauBeatriz Martín-AntonioClaudia Müller-SánchezRicardo-Pedro Casaroli-MaranoPublished in: Regenerative medicine (2020)
Aim: To determine the potential of mesenchymal stem cells (MSC) for corneal epithelial regeneration in vitro. Materials & methods: Bone marrow MSC (BM-MSC) and adipose tissue MSC were analyzed for corneal epithelial and mesenchymal markers, using limbal stem cells and corneal cells as controls. MSC with better potential were cultured with specific mediums for epithelial induction. Transepithelial electric resistance and wound healing assay with human corneal epithelial cells were performed. Results: BM-MSC showed better potential, increased corneal markers, and higher transepithelial electric resistance values when induced with limbal epithelial culture medium. Induced BM-MSC promoted better wound healing of human corneal epithelial cells by paracrine secretion. Conclusion: BM-MSC has potential for corneal epithelial induction in a protocol compatible with human application.
Keyphrases
- wound healing
- endothelial cells
- stem cells
- mesenchymal stem cells
- bone marrow
- optical coherence tomography
- adipose tissue
- high glucose
- induced pluripotent stem cells
- human health
- cataract surgery
- randomized controlled trial
- umbilical cord
- insulin resistance
- high throughput
- diabetic rats
- high fat diet
- oxidative stress
- skeletal muscle
- climate change