Low Blue Dose Photodynamic Therapy with Porphyrin-Iron Oxide Nanoparticles Complexes: In Vitro Study on Human Melanoma Cells.
Simona NistorescuAna Maria UdreaMadalina Andreea BadeaIulia LunguMihai BoniTatiana TozarFlorian DumitracheValentin Adrian MaraloiuRoua Gabriela PopescuClaudiu FleacaEcaterina AndronescuAnca DinischiotuAngela StaicuMihaela BalasPublished in: Pharmaceutics (2021)
The purpose of this study was to investigate the effectiveness in photodynamic therapy of iron oxide nanoparticles (γ-Fe 2 O 3 NPs), synthesized by laser pyrolysis technique, functionalized with 5,10,15,20-(Tetra-4-sulfonatophenyl) porphyrin tetraammonium (TPPS) on human cutaneous melanoma cells, after only 1 min blue light exposure. The efficiency of porphyrin loading on the iron oxide nanocarriers was estimated by using absorption and FTIR spectroscopy. The singlet oxygen yield was determined via transient characteristics of singlet oxygen phosphorescence at 1270 nm both for porphyrin functionalized nanoparticles and rose bengal used as standard. The irradiation was performed with a LED (405 nm, 1 mW/cm 2 ) for 1 min after melanoma cells were treated with TPPS functionalized iron oxide nanoparticles (γ-Fe 2 O 3 NPs_TPPS) and incubated for 24 h. Biological tests revealed a high anticancer effect of γ-Fe 2 O 3 NPs_TPPS complexes indi-cated by the inhibition of tumor cell proliferation, reduction of cell adhesion, and induction of cell death through ROS generated by TPPS under light exposure. The biological assays were combined with the pharmacokinetic prediction of the porphyrin.
Keyphrases
- photodynamic therapy
- iron oxide nanoparticles
- fluorescence imaging
- cell death
- endothelial cells
- cell proliferation
- quantum dots
- cell adhesion
- drug delivery
- systematic review
- energy transfer
- induced pluripotent stem cells
- iron oxide
- pluripotent stem cells
- oxide nanoparticles
- mass spectrometry
- radiation therapy
- oxidative stress
- brain injury
- blood brain barrier
- risk assessment
- cancer therapy
- heavy metals
- reactive oxygen species
- cerebral ischemia
- high speed
- liquid chromatography
- subarachnoid hemorrhage
- walled carbon nanotubes