Duck enteritis virus pUL47, as a late structural protein localized in the nucleus, mainly depends on residues 40 to 50 and 768 to 777 and inhibits IFN-β signalling by interacting with STAT1.
Tianqiong HeMingshu WangAn-Chun ChengQiao YangRenyong JiaYing WuJuan HuangShun ChenXin-Xin ZhaoMafeng LiuDekang ZhuShaqiu ZhangXuming OuSai MaoQun GaoDi SunXinJian WenBin TianYunya LiuYanling YuLing ZhangLeichang PanXiaoyue ChenPublished in: Veterinary research (2020)
Duck enteritis virus (DEV) is a member of the Alphaherpesvirinae subfamily. The characteristics of some DEV genes have been reported. However, information regarding the DEV UL47 gene is limited. In this study, we identified the DEV UL47 gene encoding a late structural protein located in the nucleus of infected cells. We further found that two domains of DEV pUL47, amino acids (aa) 40 to 50 and 768 to 777, could function as nuclear localization sequence (NLS) to guide the nuclear localization of pUL47 and nuclear translocation of heterologous proteins, including enhanced green fluorescent protein (EGFP) and beta-galactosidase (β-Gal). Moreover, pUL47 significantly inhibited polyriboinosinic:polyribocytidylic acid [poly(I:C)]-induced interferon beta (IFN-β) production and downregulated interferon-stimulated gene (ISG) expression, such as Mx and oligoadenylate synthetase-like (OASL), by interacting with signal transducer and activator of transcription-1 (STAT1).
Keyphrases
- amino acid
- genome wide identification
- genome wide
- dendritic cells
- copy number
- binding protein
- transcription factor
- immune response
- protein protein
- cell proliferation
- induced apoptosis
- genome wide analysis
- poor prognosis
- quantum dots
- herpes simplex virus
- high glucose
- dna methylation
- nuclear factor
- cell cycle arrest
- living cells
- endoplasmic reticulum stress
- small molecule
- inflammatory response
- single molecule
- stress induced
- fluorescent probe
- disease virus