Long noncoding RNA UCA1 promotes the chondrogenic differentiation of human bone marrow mesenchymal stem cells via regulating PARP1 ubiquitination.
Tao ShuJiachun LiJuyuan GuLiang WuPeng XieDongfeng ZhangWen LiJunming WanXiaozuo ZhengPublished in: Stem cells (Dayton, Ohio) (2024)
Bone marrow mesenchymal stem cells (BMSCs) possess the potential to differentiate into cartilage cells. Long noncoding RNA (lncRNAs) urothelial carcinoma associated 1 (UCA1) has been confirmed to improve the chondrogenic differentiation of marrow mesenchymal stem cells (MSCs). Herein, we further investigated the effects and underlying mechanisms of these processes. The expression of UCA1 was positively associated with chondrogenic differentiation and the knockdown of UCA1 has been shown to attenuate the expression of chondrogenic markers. RNA pull-down assay and RNA immunoprecipitation showed that UCA1 could directly bind to PARP1 protein. UCA1 could improve PARP1 protein via facilitating USP9X-mediated PARP1 deubiquitination. Then these processes stimulated the NF-κB signaling pathway. In addition, PARP1 was declined in UCA1 knockdown cells, and silencing of PARP1 could diminish the increasing effects of UCA1 on the chondrogenic differentiation from MSCs and signaling pathway activation. Collectively, these outcomes suggest that UCA1 could act as a mediator of PARP1 protein ubiquitination and develop the chondrogenic differentiation of MSCs.
Keyphrases
- mesenchymal stem cells
- long noncoding rna
- umbilical cord
- dna damage
- dna repair
- signaling pathway
- induced apoptosis
- bone marrow
- pi k akt
- poor prognosis
- cell therapy
- oxidative stress
- binding protein
- protein protein
- endothelial cells
- stem cells
- cell proliferation
- risk assessment
- endoplasmic reticulum stress
- high throughput
- high resolution
- transcription factor
- climate change
- mass spectrometry
- induced pluripotent stem cells
- network analysis