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Long-read sequencing for fast and robust identification of correct genome-edited alleles: PCR-based and Cas9 capture methods.

Christopher V McCabePeter D PriceGemma F CodnerAlasdair J AllanAdam CaulderSkevoulla ChristouJorik LoefflerMatthew MackenzieElke MalzerJoffrey MiannéKrystian J NowickiEdward J O'NeillFran J PikeMarie HutchisonBenoit Petit-DemoulièreMichelle E StewartHilary GatesSara E WellsNicholas D SandersonLydia Teboul
Published in: PLoS genetics (2024)
Targeted long-read sequencing supports in-depth characterisation of all experimental models that aim to produce knock-in or conditional alleles, including those that contain a mix of genome-edited alleles. PCR- or Cas9 capture-based modalities bring different advantages to the analysis.
Keyphrases
  • crispr cas
  • genome editing
  • single cell
  • single molecule
  • genome wide
  • optical coherence tomography
  • cancer therapy
  • real time pcr
  • gene expression