Characterization of virulence factors and antibacterial activity of curcumin in hypervirulent Klebsiella pneumoniae .
Morteza Karami-ZarandiHadi Esmaeili Gouvarchin GhalehReza RanjbarPublished in: Future microbiology (2022)
Aim: Klebsiella pneumoniae is a threat to human health due to its carbapenem-resistance and hypervirulent phenotype. Curcumin is a well-known antimicrobial agent. Hence, it is important to investigate the antivirulence activity of curcumin against hypervirulent K. pneumoniae isolates. Materials & methods: Carbapenemase presence and prevalence of hypervirulent isolates were determined. Inhibition of biofilm formation and expression of virulence genes were analyzed by colorimetry and real-time PCR tests. Results: Sixteen hypervirulent K. pneumoniae isolates were identified. The optimum activity of curcumin was detected at 1/2 minimum inhibitory concentration. Curcumin possessed appropriate antibiofilm, anti-efflux and anticapsule activities. Conclusion: According to the crucial role of biofilm, capsule and efflux systems in the pathogenesis of hypervirulent K. pneumoniae , curcumin may be used to improve anti- Klebsiella treatment.
Keyphrases
- klebsiella pneumoniae
- escherichia coli
- biofilm formation
- staphylococcus aureus
- pseudomonas aeruginosa
- multidrug resistant
- human health
- candida albicans
- risk assessment
- drug resistant
- real time pcr
- acinetobacter baumannii
- cystic fibrosis
- climate change
- poor prognosis
- genetic diversity
- gene expression
- gram negative
- risk factors
- binding protein
- dna methylation
- long non coding rna