Monitoring Changes in Intracellular Reactive Oxygen Species Levels in Response to Histone Deacetylase Inhibitors.

Oliver Holger Krämer Ramy Ashry Al-Hassan M Mustafa

Published in: Methods in molecular biology (Clifton, N.J.) (2022)

Reactive oxygen species (ROS) are induced by several chemotherapeutics. In this protocol, we describe a flow cytometry-based method for the analysis of the intracellular levels of ROS in vital leukemic cells in response to the histone deacetylase inhibitor vorinostat. This measurement of ROS using the cell-permeable dye CM-H2DCFDA indicates intracellular oxidative stress.

Keyphrases

reactive oxygen species
histone deacetylase
flow cytometry
induced apoptosis
oxidative stress
randomized controlled trial
single cell
cell cycle arrest
dna damage
cell therapy
signaling pathway
ischemia reperfusion injury
cell proliferation
bone marrow
heat stress