Early intervention in rheumatoid arthritis (RA) is critical for optimal treatment, but initiation of pharmacotherapy to prevent damage remains unsatisfactory currently. Manipulation of the gut microbiome and microbial metabolites can be effective in protecting against RA. Thus, probiotics can be utilized to explore new strategies for preventing joint damage. The aim of this study was to explore the metabolites and mechanisms by which Bifidobacterium pseudocatenulatum affects RA. Based on 16S rRNA sequencing and UPLC-MS/MS assays, we focused on bile acid (BA) metabolism. In a collagen-induced arthritis (CIA) mouse model, B. pseudocatenulatum prevented joint damage by protecting the intestinal barrier and reshaped gut microbial composition, thereby elevating bile salt hydrolase (BSH) enzyme activity and increasing the levels of unconjugated secondary BAs to suppress aberrant T-helper 1/17-type immune responses; however, these benefits were eliminated by the Takeda G protein-coupled receptor 5 (TGR5) antagonist SBI-115. The results suggested that a single bacterium, B. pseudocatenulatum , can prevent RA, indicating that prophylactic administration of probiotics may be an effective therapy.
Keyphrases
- rheumatoid arthritis
- ms ms
- disease activity
- oxidative stress
- mouse model
- ankylosing spondylitis
- immune response
- interstitial lung disease
- microbial community
- randomized controlled trial
- dendritic cells
- high glucose
- liquid chromatography tandem mass spectrometry
- single cell
- toll like receptor
- stem cells
- simultaneous determination
- combination therapy
- high resolution mass spectrometry