Differences in the Expression of TLR-2, NOD2, and NF-κB in Placenta Between Twins.
Łukasz SzylbergMagdalena BodnarAnna LebiodaPatrycja KrepskaAdam KowalewskiGrzegorz BręborowiczAndrzej MarszałekPublished in: Archivum immunologiae et therapiae experimentalis (2018)
Dizygotic twins share the same type of genetic relationship as non-twin siblings. Whereas monozygotic (MZ) twins are considered to have identical genetic material, they still differ. There is a number of reasons for early MZ twin discordance, including differences in the in utero environment, stochasticity, genetic mosaicism, and epigenetic factors. During gestation, the efficient innate immune system is of utmost importance. Our study was based on immunohistochemical evaluation of the differences in innate immune protein expression (TLR-2, NOD2, and NF-κB) in the 95 placentas between twins. Our study revealed statistical significant differences between diamniotic-dichorionic and monoamniotic-dichorionic twins. Monoamniotic-monochorionic twins exhibited no significant differences in protein expressions. To identify epigenetic factors causing the differences between twins, we made a series of comparisons with clinical data. The study revealed more cases with infections, miscarriages, in vitro fertilization, and premature rupture of membranes within the group with higher differences level of NF-κB, NOD2 and TLR-2 between twins. In case of twin-to-twin transfusion syndrome, there were no significant differences in innate immune protein expressions between twins. These results show that dissimilar genetic material and separate in utero environment promote discordance in innate immune protein expressions between twins. Moreover, additional blood flow between twins may be favorable in life-threatening conditions ensuring similar microenvironment.
Keyphrases
- innate immune
- gestational age
- immune response
- toll like receptor
- signaling pathway
- blood flow
- nuclear factor
- genome wide
- gene expression
- oxidative stress
- dna methylation
- lps induced
- poor prognosis
- protein protein
- small molecule
- copy number
- autism spectrum disorder
- deep learning
- amino acid
- electronic health record
- cell proliferation
- pi k akt
- long non coding rna
- data analysis
- acute kidney injury
- intellectual disability
- cardiac surgery