Sirt1/Foxo Axis Plays a Crucial Role in the Mechanisms of Therapeutic Effects of Erzhi Pill in Ovariectomized Rats.
Wenna LiangXihai LiGuanhui LiLiu HuShanshan DingJie KangJianying ShenCan-Dong LiTetsuya AsakawaPublished in: Evidence-based complementary and alternative medicine : eCAM (2018)
Background. Erzhi pill (EZP), a traditional Chinese herbal formula, has been widely used to treat postmenopausal osteoporosis (PMOP) in China. However, its molecular mechanisms remain unclear. The aim of the present study is to investigate the antiosteoporotic effect of EZP on an ovariectomized rat model of PMOP. We performed the biomarkers of bone metabolism disorder, bone morphology, bone mineral density (BMD), and bone biomechanics to confirm the successful establishment of the PMOP model. We then investigated the expression of biomarkers related to the Sirt1/Foxo axis. We also examined microRNA-132 (miR-132), a regulator in the Sirtuin1 (Sirt1) expression. The bone metabolism disorder, bone morphology, BMD, and bone biomechanics in ovariectomized rats were improved by EZP administration. The antiosteoporotic effect of EZP was confirmed. We also found that the expressions of Sirt1, Runx2, Foxo1, and Foxo3a were downregulated in ovariectomized rats, while being then upregulated by EZP administration. And the expression of PPAR-γ and miR-132 was upregulated in ovariectomized rats and then downregulated by EZP administration. These results provided evidence that Sirt1/Foxo axis related mechanism may play a crucial role in the therapeutic effects of EZP, indicating that Sirt1/Foxo axis can be considered as a potential therapeutic target for PMOP in the future.
Keyphrases
- bone mineral density
- postmenopausal women
- bone loss
- transcription factor
- body composition
- poor prognosis
- oxidative stress
- signaling pathway
- pi k akt
- ischemia reperfusion injury
- long non coding rna
- cell proliferation
- soft tissue
- bone regeneration
- long noncoding rna
- climate change
- risk assessment
- adipose tissue
- high resolution
- binding protein
- human health
- atomic force microscopy
- current status