An optimised clearing protocol for the quantitative assessment of sub-epidermal ovule tissues within whole cereal pistils.

Published in: Plant methods (2017)

Visualisation of cereal ovules that have not been dissected from the pistil allows an unprecedented capability to collect quantitative morphological information from the developing ovule, integument, nucellus and embryo sac. This will enable comparisons with genetic data to reveal the contribution of pre-fertilisation ovule tissues towards downstream seed development.

Keyphrases

genome wide
gene expression
randomized controlled trial
electronic health record
dna methylation
copy number
healthcare
wound healing
machine learning
deep learning