TESOGENASE, An Engineered Nuclease Editor for Enhanced Targeted Genome Integration.

Hangu NamKeqiang XieIshita MajumdarShaobo YangJakob StarzykDanna LeeRichard ShanJiahe LiHao Wu

Published in: bioRxiv : the preprint server for biology (2023)

A reporter Knock-in screening establishes enGager system to identify TESOGENASE editor to improving ssDNA mediated genome integrationMini-TESOGENASEs developed by fusing Cas9 nuclease with novel ssDNA binding motifsmRNA mini-TESOGENASEs enhance targeted genome integration via various non-viral delivery approachesEfficient functional CAR-T cell engineering by mini-TESOGENASE.

Keyphrases

crispr cas
dna binding
genome wide
cancer therapy
genome editing
dna methylation
drug delivery
gene expression
transcription factor