Single-cell chromatin accessibility and lipid profiling reveals SCD1-dependent metabolic shift in adipocytes induced by bariatric surgery.
Blaine HarlanHui Gyu ParkRoman SpektorBethany CummingsJ Thomas BrennaPaul D SolowayPublished in: PloS one (2021)
Obesity promotes type 2 diabetes and cardiometabolic pathologies. Vertical sleeve gastrectomy (VSG) is used to treat obesity resulting in long-term weight loss and health improvements that precede weight loss; however, the mechanisms underlying the immediate benefits remain incompletely understood. Because adipose plays a crucial role in energy homeostasis and utilization, we hypothesized that VSG exerts its influences, in part, by modulating adipose functional states. We applied single-cell ATAC sequencing and lipid profiling to inguinal and epididymal adipose depots from mice that received sham surgery or VSG. We observed depot-specific cellular composition and chromatin accessibility patterns that were altered by VSG. Specifically, accessibility at Scd1, a fatty acid desaturase, was substantially reduced after VSG in mature adipocytes of inguinal but not epididymal depots. This was accompanied by reduced accumulation of SCD1-produced unsaturated fatty acids. Given these findings and reports that reductions in Scd1 attenuate obesity and insulin resistance our results suggest VSG exerts its beneficial effects through an inguinal depot-specific reduction of SCD1 activity.
Keyphrases
- insulin resistance
- high fat diet induced
- single cell
- weight loss
- fatty acid
- bariatric surgery
- adipose tissue
- type diabetes
- rna seq
- metabolic syndrome
- high fat diet
- roux en y gastric bypass
- glycemic control
- skeletal muscle
- polycystic ovary syndrome
- obese patients
- gastric bypass
- high throughput
- dna damage
- gene expression
- genome wide
- minimally invasive
- transcription factor
- radical prostatectomy
- weight gain
- cardiovascular disease
- public health
- climate change
- acute coronary syndrome
- signaling pathway
- risk assessment
- oxidative stress
- social media
- health promotion