Gas chromatography/mass spectrometry analysis of organic acid profiles in human serum: A protocol of direct ultrasound-assisted derivatization.

Ming-Jia ZhangJing ChouZhi-Wei SunJin-Hui ZhaoJing GuoJia-Ying YuSi-Qi GaoYing-Shu TangLi-Yan Liu

Published in: Rapid communications in mass spectrometry : RCM (2021)

We identified and validated a protocol for ultrasound-assisted derivatization prior to GC/MS analysis for detecting 26 kinds of organic acids in serum. The results suggest the efficacy of this protocol for clinical applications to determine metabolic changes related to fluctuations in organic acid profiles.

Keyphrases

gas chromatography mass spectrometry
randomized controlled trial
solid phase extraction
gas chromatography
ms ms
high performance liquid chromatography
liquid chromatography tandem mass spectrometry
water soluble
simultaneous determination
liquid chromatography
tandem mass spectrometry