Small RNA F6 Provides Mycobacterium smegmatis Entry into Dormancy.
Artem GrigorovOksana BychenkoElena G SalinaYulia SkvortsovaArina MazurovaTimofey SkvortsovArseny KaprelyantsTatyana L AzhikinaPublished in: International journal of molecular sciences (2021)
Regulatory small non-coding RNAs play a significant role in bacterial adaptation to changing environmental conditions. Various stresses such as hypoxia and nutrient starvation cause a reduction in the metabolic activity of Mycobacterium smegmatis, leading to entry into dormancy. We investigated the functional role of F6, a small RNA of M. smegmatis, and constructed an F6 deletion strain of M. smegmatis. Using the RNA-seq approach, we demonstrated that gene expression changes that accompany F6 deletion contributed to bacterial resistance against oxidative stress. We also found that F6 directly interacted with 5'-UTR of MSMEG_4640 mRNA encoding RpfE2, a resuscitation-promoting factor, which led to the downregulation of RpfE2 expression. The F6 deletion strain was characterized by the reduced ability to enter into dormancy (non-culturability) in the potassium deficiency model compared to the wild-type strain, indicating that F6 significantly contributes to bacterial adaptation to non-optimal growth conditions.
Keyphrases
- rna seq
- gene expression
- wild type
- single cell
- oxidative stress
- mycobacterium tuberculosis
- cardiac arrest
- poor prognosis
- dna methylation
- dna damage
- signaling pathway
- cell proliferation
- transcription factor
- endothelial cells
- wastewater treatment
- climate change
- atomic force microscopy
- ischemia reperfusion injury
- endoplasmic reticulum stress
- mass spectrometry
- high speed