Modified PCR protocol to increase sensitivity for determination of bacterial community composition.
Kayla M WilliamsonBrandie D WagnerCharles E RobertsonMark J StevensMarci K SontagPeter M MouraniJ Kirk HarrisPublished in: Microbiome (2021)
The modified amplification protocol represents a viable approach that increased sensitivity of bacterial community analysis, which is important for study of the human airway microbiome where bacterial load is highly variable. Video abstract.