Melittin from Apis florea Venom as a Promising Therapeutic Agent for Skin Cancer Treatment.
Sirikwan SangboonruangKuntida KitideePanuwan ChantawannakulKhajornsak TragoolpuaYingmanee TragoolpuaPublished in: Antibiotics (Basel, Switzerland) (2020)
Melittin, a major component found in bee venom, is produced by the Apis species of the honey bee. In this study, the effect of melittin derived from Apis florea (Mel-AF), which is a wild honey bee species that is indigenous to Thailand, was investigated against human malignant melanoma (A375) cells. In this study, Mel-AF exhibited considerable potential in the anti-proliferative action of A375 cells. Subsequently, the cellular mechanism of Mel-AF that induced cell death was investigated in terms of apoptosis. As a result, gene and protein expression levels, which indicated the activation of cytochrome-c release and caspase-9 expression, eventually triggered the release of the caspase-3 executioner upon Mel-AF. We then determined that apoptosis-mediated cell death was carried out through the intrinsic mitochondrial pathway. Moreover, advanced abilities, including cell motility and invasion, were significantly suppressed. Mel-AF manipulated the actin arrangement via the trapping of stress fibers that were found underneath the membrane, which resulted in the defective actin cytoskeleton organization. Consequently, the expression of EGFR, a binding protein to F-actin, was also found to be suppressed. This outcome strongly supports the effects of Mel-AF in the inhibition of progressive malignant activity through the disruption of actin cytoskeleton-EGFR interaction and the EGFR signaling system. Thus, the findings of our current study indicate the potential usefulness of Mel-AF in cancer treatments as an apoptosis inducer and a potential actin-targeting agent.
Keyphrases
- cell death
- cell cycle arrest
- atrial fibrillation
- small cell lung cancer
- induced apoptosis
- oxidative stress
- binding protein
- cell migration
- poor prognosis
- epidermal growth factor receptor
- tyrosine kinase
- pi k akt
- endothelial cells
- mass spectrometry
- young adults
- multiple sclerosis
- single cell
- high glucose
- copy number
- risk assessment
- squamous cell carcinoma
- mesenchymal stem cells
- cystic fibrosis
- biofilm formation
- papillary thyroid
- pseudomonas aeruginosa
- candida albicans