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Comprehensive benchmarking of CITE-seq versus DOGMA-seq single cell multimodal omics.

Zhongli XuElisa Heidrich-O'HareWei ChenRichard H Duerr
Published in: Genome biology (2022)
The recently developed method TEA-seq and similar DOGMA-seq single cell trimodal omics assays provide unprecedented opportunities for understanding cell biology, but independent evaluation is lacking. We explore the utility of DOGMA-seq compared to the bimodal CITE-seq assay in activated and stimulated human peripheral blood T cells. We find that single cell trimodal omics measurements after digitonin (DIG) permeabilization were generally better than after an alternative "low-loss lysis" (LLL) permeabilization condition. Next, we find that DOGMA-seq with optimized DIG permeabilization and its ATAC library provides more information, although its mRNA and cell surface protein libraries have slightly inferior quality, compared to CITE-seq.
Keyphrases
  • single cell
  • rna seq
  • high throughput
  • peripheral blood
  • endothelial cells
  • cell surface
  • healthcare
  • gene expression
  • binding protein
  • mesenchymal stem cells
  • small molecule
  • social media
  • pluripotent stem cells