Mini-XT, a miniaturized tagmentation-based protocol for efficient sequencing of SARS-CoV-2.

Marc FuchsClara RadulescuMiao TangArun MaheshDeborah LavinSyed UmbreenJames McKennaMark SmythEilís McColganZoltan MolnarChris BaxterTimofey SkvortsovAditi SinghFiona RoganJulia MiskellyStephen BridgettDerek FairleyDavid A Simpson

Published in: Journal of translational medicine (2022)

The Mini-XT protocol maintains sequence quality while reducing library preparation reagent volumes eightfold and halving overall tip usage from sample to sequence to provide concomitant cost savings relative to standard protocols. This will enable more efficient high-throughput sequencing of SARS-CoV-2 isolates and future pathogen WGS.

Keyphrases

sars cov
high throughput sequencing
respiratory syndrome coronavirus
randomized controlled trial
single cell
current status
amino acid
candida albicans
quality improvement
high resolution