Optimization of small RNA library preparation protocol from human urinary exosomes.

Dolores OlivaresJavier Perez-HernandezDaniel Perez-GilFelipe J ChavesJosep RedonRaquel Cortes

Published in: Journal of translational medicine (2020)

This study provides researchers with an optimized small RNA library preparation workflow for next generation sequencing based exosome-associated miRNA analysis that yields a high amount of miRNA mapped reads without skewing the tagged miRNA population significantly.

Keyphrases

endothelial cells
molecularly imprinted
stem cells
mesenchymal stem cells
randomized controlled trial
nucleic acid
induced pluripotent stem cells
electronic health record
copy number
high resolution
genome wide
circulating tumor cells