Impaired Inflammatory Response to LPS in Type 2 Diabetes Mellitus.
Lusine KhondkaryanSona MargaryanDavid PoghosyanGayane ManukyanPublished in: International journal of inflammation (2018)
Type 2 diabetes mellitus (T2DM) is a severe health problem worldwide, reaching epidemic levels. High susceptibility to infections of T2DM patients indicates dysregulated immune responses to pathogens. However, innate immune responses, including monocyte functions, in T2DM are poorly investigated. Therefore, in this study we aimed to assess lipopolysaccharide- (LPS-) induced immune responses of circulating monocytes from T2DM patients. The results showed that monocytes from T2DM were hyporesponsive to LPS challenge as reflected by significantly suppressed secretion of TNFα (p < 0.01) and expression of CD11b (p < 0.001) and TLR4 (p < 0.001) compared to those in monocytes from healthy subjects. Furthermore, LPS-induced IL-10 levels were similar in diabetic and healthy supernatants, while expression levels of CD163 were found to be downregulated on monocytes from T2DM (p < 0.001) suggesting impaired ability of monocytes to switch their phenotype to anti-inflammatory. Taken together, our results suggest compromised function of monocytes in T2DM, which may explain, at least partly, high incidence of infection in these patients.
Keyphrases
- inflammatory response
- lps induced
- immune response
- end stage renal disease
- dendritic cells
- chronic kidney disease
- toll like receptor
- newly diagnosed
- ejection fraction
- peripheral blood
- anti inflammatory
- type diabetes
- lipopolysaccharide induced
- peritoneal dialysis
- public health
- glycemic control
- prognostic factors
- poor prognosis
- healthcare
- cardiovascular disease
- risk factors
- mental health
- rheumatoid arthritis
- multidrug resistant
- gram negative
- long non coding rna
- social media
- cardiovascular risk factors
- patient reported
- wound healing