Metabolomics and Lipidomics Profiling of a Combined Mitochondrial Plus Endoplasmic Reticulum Fraction of Human Fibroblasts: A Robust Tool for Clinical Studies.
Charlotte Veyrat-DurebexCinzia BoccaStéphanie ChupinJudith Kouassi NzoughetGilles SimardGuy LenaersPascal ReynierHélène BlascoPublished in: Journal of proteome research (2017)
Mitochondria and endoplasmic reticulum (ER) are physically and functionally connected. This close interaction, via mitochondria-associated membranes, is increasingly explored and supports the importance of studying these two organelles as a whole. Metabolomics and lipidomics are powerful approaches for the exploration of metabolic pathways that may be useful to provide deeper information on these organelles' functions, dysfunctions, and interactions. We developed a quick and simple experimental procedure for the purification of a mitochondria-ER fraction from human fibroblasts. We applied combined metabolomics and lipidomics analyses by mass spectrometry with excellent reproducibility. Seventy-two metabolites and 418 complex lipids were detected with a mean coefficient of variation around 12%, among which many were specific to the mitochondrial metabolism. Thus this strategy based on robust mitochondria-ER extraction and "omics" combination will be useful for investigating the pathophysiology of complex diseases.
Keyphrases
- endoplasmic reticulum
- mass spectrometry
- endothelial cells
- liquid chromatography
- oxidative stress
- induced pluripotent stem cells
- pluripotent stem cells
- gas chromatography
- extracellular matrix
- high performance liquid chromatography
- ms ms
- magnetic resonance imaging
- computed tomography
- cell death
- genome wide analysis